The positive sera against neutralizing epitopes on the common ancestor of adenoviral hexon were relatively rare among healthy adults.Ĭonclusion: The existing strains inferred the common ancestor of human adenoviruses, with epitopes never observed in the current human strains. The modern strains successfully predicted the common ancestral sequence of the human adenovirus hexon. Results: The tower region of the hexon protein had the highest sequence variability, while the neck and base regions remained constant among different types. The immunological features of ancestral loop 1 and loop 2 regions of sequences were tested using protein segments expressed in a prokaryotic expression system and polypeptides synthesized with human serum samples.
The three-dimensional structure model was built using homology modeling methods. Methods: The sequences of 74 adenovirus-type strains were used to predict the ancestral sequence of human adenovirus hexon protein using FastML and MEGA software. And to provide a candidate hexon protein for developing new adenoviral vector capable of escaping the pre-existing immunity in healthy populations.
Yingchen Wang 1†, Zhe Zhang 1†, Lei Shang 1, Hong Gao 1, Xiqiao Du 1,2, Falong Li 1, Ya Gao 1, Guiyun Qi 3, Weiyuan Guo 3, Zhangyi Qu 1,4* and Tuo Dong 1*
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